N643
RIBOZOL™ PLUS RNA PURIFICATION KIT

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None
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Storage Condition:
ROOM TEMPERATURE
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NONE
Code
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N643-KIT

KIT

$306.00

Generate high yields of purified, concentrated total RNA with a simple 2 part procedure that combines an initial extraction step with RiboZol™  RNA Extraction Reagent followed by spin column chromatography for additional purification and concentration.
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Material Safety Data Sheet
Certificate of Analysis
Directions for Use

RiboZol™ Plus RNA Purification Kit

Agarose gel electrophoresis of RiboZol Plus purified RNA
A simple 2 part RNA purification procedure that combines an initial extraction step with RiboZol™  RNA Extraction Reagent followed by spin column chromatography to generate high yields of purified, concentrated total RNA.
  • The proprietary spin column matrix binds RNA while the unwanted protein and DNA are washed through.
  • RNA is eluted with supplied Elution Buffer.
    • Excellent recovery of large RNAs such as rRNA and mRNA.
    • Superior recovery of small RNAs including miRNA and siRNA.
    • Compatible with downstream applications including qRT-PCR.
    • RiboZol™ Plus Kit enhances recovery of small RNA species. 5x106 K562 cells were isolated with RiboZol™ RNA Extraction Reagent, and further purified with RiboZol™ Plus Purification Kit Columns (lane 2-3) or Competitor’s Purification Kit Columns (lanes 4-5). Arrow indicates small RNA species that are recovered in greater abundance with the columns used in the RiboZol™ Plus RNA Purification Kit compared to Competitor’s Kit columns.

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    Compatiblity with downstream applications

    RT-qPCR of Ribozol Plus purified RNA templates
    RT-qPCR using miR-21 and 5S rRNA primers. Amplification of 5S rRNA and miR-21 RNA purifiied with RiboZol™ Plus. RNA from 1x106 HeLa cells was extracted and purified using RiboZol ™ Plus RNA Purification Kit. 2μl RNA was used in a 20μl RT reaction with either a 5S reverse primer or miR-21 stem-loop reverse primer. 2μl of the RT reaction was analyzed by qPCR using BioRad’s iQ™ SYBR® Green Master mixes.

    Simple and Rapid Procedure


    RiboZol™ Plus RNA Purification Kit Components

    RiboZol Plus RNA Purification Kit components
    Each kit includes sufficient RiboZol RNA Extraction Reagent, spin columns and collection tubes for 50 isolations

    Related products

    RiboZol™ RNA Extraction Reagent

     Ready-to-use, single phase RNA extraction reagent
    • High Yields
    • High Purity

    RNA Storage Buffers
    AMRESCO offers two RNA storage buffers that insure the stability of purified RNA during extended storage. These buffers are rigorously tested for contaminants that promote RNA degredation and are guaranteed free of RNase activity.
    RiboReserve™ RNA Storage Buffer
    1 mM Sodium Citrate, pH 6.4
    TE, pH 7.0

    Storage buffers are available in 2 packaging options:
    • 50 ml bottles
    • Convenient 1 ml tubes -12 tubes per package
    Electrophoresis (P/F)
    PASS