N643
RIBOZOL™ PLUS RNA PURIFICATION KIT

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None
Molecular Weight:
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Storage Condition:
ROOM TEMPERATURE
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Hazard Code:
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NONE
Code
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Price
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N643-KIT

KIT

$347.73

Overview

Generate high yields of purified, concentrated total RNA with a simple 2 part procedure that combines an initial extraction step with RiboZol™ RNA Extraction Reagent followed by spin column chromatography for additional purification and concentration.

  • Excellent recovery of large RNAs such as rRNA and mRNA.
  • Superior recovery of small RNAs including miRNA and siRNA.
  • Compatible with downstream applications including qRT-PCR

How to Use

Click on the Features tab to learn more about RiboZol™ Plus RNA Purification Kit



Material Safety Data Sheet
Certificate of Analysis
Directions for Use

RiboZol™ Plus RNA Purification Kit

RiboZol™ Plus RNA Purification Kit purifies and recovers all sizes and species of RNA, including large RNAs such as ribosomal RNA (rRNA) or messenger RNA (mRNA), and small RNAs less than 200 nucleotides, including microRNAs (miRNA) and small inhibitory RNAs (siRNA).  The kit combines the benefits of organic RNA extraction using RiboZol™ RNA Extraction Reagent with the ease and convenience of spin column technology.  First, RiboZol™ RNA Extraction Reagent extracts total RNA from cells or tissue, including difficult sample types.  Subsequent column chromatography employs a proprietary resin as the separation matrix to yield up to 35 µg of highly purified total RNA.  

Total RNA purified with RiboZol™ Plus RNA Purification Kit is compatible with most downstream applications including Northern blot and dot blot analysis, RNase protection assays, microarray analysis, molecular cloning and mRNA isolation.  DNase treatment is recommended for maximum removal of DNA prior to sensitive downstream applications such as quantitative RT-PCR.

RiboZol™ Plus Kit enhances recovery of small RNA species

Agarose gel electrophoresis of RiboZol Plus purified RNA
RNA from K562 cells (5x106) were isolated with RiboZol™ RNA Extraction Reagent, and further purified with RiboZol™ Plus Purification Kit Columns (lane 2-3) or Competitor’s Purification Kit Columns (lanes 4-5). Arrow indicates small RNA species that are recovered in greater abundance with the columns used in the RiboZol™ Plus RNA Purification Kit compared to Competitor’s Kit columns.


RiboZol™ Plus is compatible with RT-qPCR using miR-21 and 5S rRNA primers.

RT-qPCR of Ribozol Plus purified RNA templates
Amplification of 5S rRNA and miR-21 RNA purifiied with RiboZol™ Plus. RNA from 1x106 HeLa cells was extracted and purified using RiboZol ™ Plus RNA Purification Kit. 2μl RNA was used in a 20μl RT reaction with either a 5S reverse primer or miR-21 stem-loop reverse primer. 2μl of the RT reaction was analyzed by qPCR using BioRad’s iQ™ SYBR® Green Master mixes.

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Electrophoresis (P/F)
PASS