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ISO 9001 & ISO 13485 Certified

1B1304
RIBOZOL™ ME (MICRO ENRICHED)

Grade:

CAS Number:
N/A
Synonym(s):
rna extraction reagent
Molecular Weight:
N/A
Molecular Formula:
N/A
Storage Condition:
ROOM TEMPERATURE
Risk Statements:
36/37/38-42/43-20/21/22
Safety Statements:
36/37/39
Hazard Code:
irritant harmful sensitizer
UN Number:
Code
Size
Price
Quantity
1B1304-30ML

30ML

$52.50

1B1304-100ML

100ML

$125.00

1B1304-200ML

200ML

$229.55

Overview

  • Easy-to-use
  • Works with a variety of cell and tissue types
  • Enriches for small MW RNAs
  • Reduces the concentration of large MW RNAs

Detailed Description

RiboZol™ ME (Micro Enriched) is a variation on AMRESCO's widely popular RiboZol™ RNA Extraction Reagent. Like its predecessor, RiboZol™ ME follows a simple protocol whereby a variety of cell and tissue types can be homogenized directly in the single phase phenol solution, minimizing degradation of RNA by RNAses. In contrast to RiboZol™ RNA Extraction Reagent, RiboZol™ ME is specially formulated to enrich the RNA sample with small molecular weight RNAs. RiboZol™ ME also reduces the concentration of large molecular weight RNAs, providing the researcher with a RNA sample ideal for miRNA discovery.

Click on the Features tab to learn more about RiboZol™ ME (Micro Enriched)


Material Safety Data Sheet
Certificate of Analysis
Directions for Use

Amplification of miR-21 RNA from Total RNA purified with RiboZol™ or  RiboZol™ ME.  Total RNA from 1x106 HeLa cells was extracted and purified using RiboZol™ RNA Extraction Reagent [green arrow] (N580) or RiboZol™ ME,[red arrow]  (1B1304). Purified RNA (0.75µg) was reverse transcribed in a 20μl reaction with miR-21 stem-loop reverse primer. The RT reaction (3 µL) was analyzed by qPCR using BioRad’s iQ™ SYBR® Green Master mixes.

Enrichment of small RNAs using RiboZol ME. Total RNA was extracted in triplicate from K562 cells (2.79x106 cells) with  RiboZol™ ME (1B1304). RNA pellets were resuspended in RiboReserve™ RNA Storage Solution (N633). Heat-denatured RNA samples (10µg) from both purifications were electrophoresed on a 2% Agarose I™ (0710) formaldehyde gel.