AMRESCO's ZYP-5052, Autoinduction Media is formulated with quality biochemicals to ensure consistent growth and protein expression using methodology developed by Dr. Studier at Brookhaven National Laboratory. ZYP-5052, Autoinduction Media activates recombinant protein expression in E. coli through diauxic growth, with the first growth phase producing a high density bacterial culture. During the second phase, lac promoters are activated and result in induction of prolific transcription and translation of the cloned DNA. A significant percentage of the total cell protein becomes comprised of recombinant protein, which can then be isolated and purified for downstream applications.
A protein expression protocol using ZYP-5052, Autoinduction Media is more effiecient than conventional methods for both high throughput and routine protein expression, because it eliminates the need to monitor cell density and the manual addition of IPTG for induction. An additional benefit of using ZYP-5052, Autoinduction Media is improved protein yield compared to the yield achieved by a conventional growth and induction method.
CONTACT US
Phone
Toll-Free: 800.448.4442
(U.S. & Canada)
Local: 440.349.1199
Technical Support
Toll-free: 800.610.2789
FAX
Local FAX: 440.349.1182
General Information
Customer Service
Website Questions
Technical Support
Mailing Address
AMRESCO LLC
6681 Cochran Road
Solon, OH 44139 USA