Preserve RNA Integrity
Homogenize samples in this single phase phenol solution, minimizing the degradation of RNA by RNAses.
Ideal for miRNA
Discovery Formulated to enrich the RNA sample with small molecular weight RNAs and reduce the concentration of large molecular weight RNAs.
Versatile
Works with variety of cell and tissue types is compatible with various downstream applications, such as Northern blots and RT/PCR.
Amplification of miR-21 RNA from Total RNA purified with RiboZol™ or RiboZol™ ME. Total RNA from 1x106 HeLa cells was extracted and purified using RiboZol™ RNA Extraction Reagent [green arrow] (N580) or RiboZol™ ME,[red arrow] (1B1304). Purified RNA (0.75µg) was reverse transcribed in a 20μl reaction with miR-21 stem-loop reverse primer. The RT reaction (3 µL) was analyzed by qPCR using BioRad’s iQ™ SYBR® Green Master mixes.
Enrichment of small RNAs using RiboZol ME. Total RNA was extracted in triplicate from K562 cells (2.79x106 cells) with RiboZol™ ME (1B1304). RNA pellets were resuspended in RiboReserve™ RNA Storage Solution (N633). Heat-denatured RNA samples (10µg) from both purifications were electrophoresed on a 2% Agarose I™ (0710) formaldehyde gel.
RiboZol Plus RNA Purification Kit (N643)
NEW Rapid Formaldehyde-Free RNA Gel Kit (1B1384)
RiboReserve RNA Storage Solution (N633)
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