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Ready One-Step RT-PCR Kit, 2X

Streamlined procedure allows you to load samples directly to gel after PCR reaction and visualize in one step

Ordering Information

Convenient Format
Fast single-tube setup radically reduces prep and processing time. All-in-one master mix includes high-fidelity polymerase blend, reaction buffer, dNTPs, tracking dye, and non-mutagenic visualization dye.

One-Step Process
Single step procedure dramatically reduces time spent preparing PCR reaction.

Instant Visualization
Fluorescent visualization dye included in the solution allows for immediate visualization post-electrophoresis with standard UV illumination.

Kit Components

  • 1 tube of Ready One-Step Reverse Transcriptase
  • 1 or 2 tubes of Ready One-Step RT-PCR Mix, 2X

Detection down to one pg of RNA. DNase I (0649) treated RNA purified from 1x107 K562 cells with RiboZol (N580) was serial diluted in PCR tubes.  Forward and Reverse Primers for ß-actin were added to the RNA templates. The nucleic acid solutions were heat-denatured at 65°C for 5 minutes and immediately placed on ice.  Components of the Ready One-Step RT-PCR Kit were added to the RT-PCR reactions and a conventional RT-PCR cycle was used to amplify DNA.  The product was separated on a 1% agarose gel in TAE buffer.



Eliminates the need for Ethidium bromide. DNase I (0649) treated RNA (1µg) purified from 1x107 K562 cells with RiboZol (N580) was placed in a PCR tube.  Forward and Reverse Primers for GAPDH were added to the RNA template. The nucleic acid solution was heat-denatured at 65°C for 5 minutes and immediately placed on ice.  Components of the Ready One-Step RT-PCR Kit were added to the RT-PCR reaction and a conventional RT-PCR cycle was used to amplify DNA.  The product was separated on a 1% agarose gel in TAE buffer and immediately visualized on a Syngene HR gel doc system.

Click here to download the Ready One-Step RT-PCR Kit product flyer.

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  • Purifies and recovers all sizes and species of RNA

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  • RNA extraction yields from a variety of cell lines

EZ-Vision® PCR DNA Ladder (N853) 

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