Convenient Format
Fast single-tube setup radically reduces prep and processing time. All-in-one master mix includes high-fidelity polymerase blend, reaction buffer, dNTPs, tracking dye, and non-mutagenic visualization dye.
One-Step Process
Single step procedure dramatically reduces time spent preparing PCR reaction.
Instant Visualization
Fluorescent visualization dye included in the solution allows for immediate visualization post-electrophoresis with standard UV illumination.
Kit Components
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DNase I (0649) treated RNA purified from 1x107 K562 cells with RiboZol (N580) was serial diluted in PCR tubes. Forward and Reverse Primers for ß-actin were added to the RNA templates. The nucleic acid solutions were heat-denatured at 65°C for 5 minutes and immediately placed on ice. Components of the Ready One-Step RT-PCR Kit were added to the RT-PCR reactions and a conventional RT-PCR cycle was used to amplify DNA. The product was separated on a 1% agarose gel in TAE buffer.
DNase I (0649) treated RNA (1µg) purified from 1x107 K562 cells with RiboZol (N580) was placed in a PCR tube. Forward and Reverse Primers for GAPDH were added to the RNA template. The nucleic acid solution was heat-denatured at 65°C for 5 minutes and immediately placed on ice. Components of the Ready One-Step RT-PCR Kit were added to the RT-PCR reaction and a conventional RT-PCR cycle was used to amplify DNA. The product was separated on a 1% agarose gel in TAE buffer and immediately visualized on a Syngene HR gel doc system.
Phenol-Free Total RNA Purification Kit (N788)
RiboZol Plus RNA Purification Kit (N643)
RiboZol RNA Extraction Reagent (N580)
EZ-Vision® PCR DNA Ladder (N853)
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