Convenient premixed loading buffer for use in Laemmli SDS-PAGE gels
Ordering Information
Laemmli Loading Buffer, 4X is formulated for use in Laemmli SDS-PAGE, a widely used system for protein electrophoresis. Mixing Laemmli Loading Buffer, 4X with samples reduces disulfide bonds and denatures the proteins due to the buffer's 2-mercaptoethanol and SDS detergent content. The binding of SDS to denatured protein imparts uniform negative charge density so that separation onthe gel is based on size. Glycerol is included in the loading buffer as a density agent to ensure the samples sink into the sample wells, while bromophenol blue serves as tracking dye to monitor electrophoretic progression.
