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ISO 9001 & ISO 13485 Certified

BCS Assay Kit

Spectophotometric determination of protein concentration

Ordering Information

Uniform Detection
Protein concentration is inversely proportional to the concentration of peptide bonds as opposed to specific amino acid side chains. This results in less signal variation across different protein samples.

Highly Compatible
BCS assay has a high tolerance for salts, detergents, and buffers that may interfere with conventional protein quantitation kits.

Sensitive Response
Utilizes a reaction where copper(II) binds the protein. Free copper(II) is reduced to copper(I) which associates with dye. Detection is sensitive to 5ug/mL protein.

Kit Components

  • 10 ml or 50 ml of BCS Assay, Solution A
  • 90 ml or 450 ml of BCS Assay, Solution B

Covers a broader protein concentration range than Bradford

Standard curve linear ranges for Bradford Reagent and BCS Assay Kit. BSA standards (5, 10, 20, 30, 40, 50µg) were prepared in triplicate and mixed with either Bradford reagent [] or AMRESCO’s BCS Assay Kit [] (N962). Absorbances were read and standard curves were plotted. Error bars represent one standard deviation.

Higher tolerance for detergents than other assays

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