SeraFree™ Cryopreservation Media

SeraFree™ Cryopreservation Media (RPMI)
Code # N655

SeraFree™ DMEM Cryopreservation Media
Code # N676

Ready-to-use freezing media for cryopreservation of adherent or suspension cultured cells
  • Animal-free RPMI or DMEM based defined media composition eliminates batch-to-batch variability
  • Optimizes cell viability and cell growth after thawing
  • Sterile and endotoxin tested
  • Reduces potential for transmission of infectious agents
  • Eliminates regulatory compliance requirements associated with use of animal products
  • Cost effective

K562 Cell Post-thaw Viability Frozen in SeraFree™ Media versus Serum Containing Media

SeraFree Cryoprotection Media
Viability of post-thaw cultures of K562 cells. Cells frozen in AMRESCO SeraFree™ Cryoprotection Media exhibited higher recovery than cells frozen in traditional RPMI freezing media containing DMSO and serum. Viability of cells recovering from cryopreservation in AMRESCO SeraFree™ Cryoprotection Media was comparable or better than that of cells in Competitors 1 and 2 serum-free freezing media. Viability was determined by standard trypan-blue exclusion assay.

HeLa Cell Viability after Storage in SeraFree™ Media vs Serum Containing Media

SeraFree Cryopreservation Media post-thaw viability
Performance of AMRESCO SeraFree™ Cryopreservation Media rival serum-containing media. HeLa cells (1x106) were frozen in  AMRESCO’s DMEM or RPMI SeraFree™ media,   serum-containing freezing  media and a competitor’s serum-free freezing media. Thawed cells were counted using standard trypan blue exclusion assay.

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