RiboZol™ RNA Extraction Reagent

RiboZol RNA Extraction Reagent Gel1

RiboZol™ RNA Extraction Reagent
Code: N580

Ready-to-use, single phase RNA extraction reagent
  • High Yields of total RNA
  • Rapid Procedure: Complete extraction in < 1 hour
  • Excellent results from even the most difficult of cells and tissues
  • Compatible with a variety of downstream applications:
    • Northern analysis
    • Dot blots
    • Cloning
    • In vitro translation of  PolyA+ selected RNA
    • RT-PCR
Figure 1. RiboZol™ Extracted RNA. Total RNA was extracted from K562 cells (1x106 cells) with RiboZol™ RNA Extraction Reagent. RNA pellets were resuspended in RiboReserve™ RNA storage buffer. Samples were electrophoresed on a 1.2% Agarose I™ formaldehyde gel. Lane 1: RiboReady™ 1 Kb RNA Ladder; Lanes 2 & 3: RiboZol™ extracted RNA samples.

High yields of total RNA

Yields of RNA extracted with RiboZol™ are comparable to those obtained with a competitor’s extraction reagent.
Figure 2. RiboZol™ extracted RNA yields from 2 different cell lines. Data provided by Eunmi Ho, Catholic University, Korea. RNA was extracted from HeLa cell cultures (purple bars) 1x106 cells and from CaSki cultures (magenta bars) 1x106 cells according to standard procedures. Total RNA yields were determined by OD260

Excellent purity

Purity of RNA extracted with RiboZol™ are comparable to those obtained with a competitor’s extraction reagent
Figure 3. Purity of RiboZol™ extracted RNA from 2 different cell lines. Data provided by Eunmi Ho, Catholic University, Korea. RNA was extracted from HeLa cell cultures (purple bars) 1x106 cells and from CaSki cultures (magenta bars) 1x106 cells according to provided procedures. RNA purity was determined by the OD260/280 ratio.

Compatibility with downstream applications

Tests of RiboZol™ extracted mRNA confirm that it is a fully suitable template for cDNA synthesis. In side-by-side comparisons, RiboZol™ extracted mRNA equaled results obtained with mRNA extracted with a competitor’s reagent.
cDNA synthesis of Ribozol extracted RNA
Figure 4. cDNA synthesis/PCR from RiboZol™ extracted RNA. Data provided by Beth Sullivan, PhD. Duke University Medical Center, Durham, NC. cDNA synthesis: mRNA was extracted from GM08854-mouse/ Human Chr21 hybrid cells with RiboZol™ or with a competitor’s reagent. 2 μg samples were added to SuperScript®III First Strand Synthesis System (Invitrogen) as templates for reverse transcription. PCR reactions: 0.5 μl of each cDNA was added to PCR reactions containing primers to mouse beta actin. Samples were removed after 35 cycles and loaded onto 2% agarose gels. Lane 1: 100 bp ladder; Lane 2: water; Lane 3: RiboZol™ RNA - RT; Lane 4: RiboZol™ RNA + RT; Lane 5: Competitor RNA - RT; Lane 6: Competitor RNA + RT.

Related Products

RiboZol™ Plus Total RNA Purification Kit
RiboZol™ Extraction Reagent + Spin Column Purification
  • Purify and concentrate RNA in < 1 hour
  • Enrich for small RNA species

RNA Storage Buffers
AMRESCO offers two RNA storage buffers that insure the stability of purified RNA during extended storage. These buffers are rigorously tested for contaminants that promote RNA degredation and are guaranteed free of RNase activity.
RiboReserve™ RNA Storage Buffer
1 mM Sodium Citrate, pH 6.4
TE, pH 7.0
Storage buffers are available in 2 packaging options:
  • 50 ml bottles
  • Convenient 1 ml tubes -12 tubes per package

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