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Rapid Formaldehyde-Free RNA Gel Kit

Safer alternative to formaldehyde-containing agarose gels now with NEW Rapid RNA Gel Running Buffer included

Ordering Information

Rapid Results
Specially formulated Rapid RNA Gel Running Buffer enables electrophoresis at higher voltages thereby reducing electrophoresis time in half.

Eliminate Toxic Chemicals in the Lab
The only RNA gel kit free of ethidium bromide and formaldehyde. Hood-free pouring and running allows you to run gels directly on your benchtop.

Immediate Visualization
Non-hazardous RNA dye included in the sample buffer stains RNA bands a brilliant green upon UV illumination after electrophoresis. No poststaining required.

Downstream Compatibility
Fully compatible with downstream applications such as Northern blots.

Kit Components

  • Rapid Formaldehyde-Free RNA Gel Solution, 10X, 150 ml
  • Rapid RNA Gel Running Buffer, 10X, 2 x 500 ml
  • Formaldehyde-Free RNA Gel Loading Buffer, 2X, 3 x 2 ml
Formaldehyde-Free RNA Gel Kit

Rapid Formaldehyde-Free RNA dye versus ethidium bromide staining

Total RNA was extracted from K562 cells with Ribozol® RNA Extraction Reagent (N580). Samples were denatured in Rapid Formaldehyde-Free RNA Loading Buffer containing either ethidium bromide or Rapid Formaldehyde-Free RNA dye and incubated for 10 minutes at 65°C. The samples were applied to a 2% Formaldehyde-Free RNA Agarose Gel (Agarose I™, Code:0710) and resolved for 1.5 hours at 5.1 V/cm. Image capture was performed with a Syngene GBox-HR Gel Doc System with a SYBR® Green filter.
Norther Blot of Formaldehyde Free RNA Gel

Northern Blot Analysis of PGK1 mRNA

Northern blot analysis of PGK1 mRNA resolved on Formaldehyde-Free RNA Gel. Data provided by Jeff Coller, Ph.D., Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, Ohio. Gel A: 20 μg of total yeast RNA was applied per lane to an agarose Formaldehyde-Free RNA Gel and resolved at 8V/cm. Bands were visualized on a UV transilluminator Gel B: Bands were transferred to Hybond N membrane and probed overnight with a radiolabelled oligonucleotide to PGK1 mRNA. Gel C: Membranes were stripped and reprobed over night with an oligonucleotide to SCR1 RNA loading control.
Lane 1: Untransformed control
Lane 2: High Copy PGK1 vector
Lane 3: Low Copy PGK1 vector
Lane 4: High Copy PGK1 vector
Lane 5: Low Copy PGK1 vector

Related Products

Phenol-Free Total RNA Purification Kit (N788)

  • Purify high quality RNA in just 20 minutes

RiboZol™ Plus RNA Purification Kit (N643)

  • Purifies and recovers all sizes and species of RNA

RiboZol™ RNA Extraction Reagent (N580)

  • RNA extraction yields from a variety of cell lines

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