RNA EZ-Vision Dye as Loading Buffer


A non-mutagenic alternative to ethidium bromide for RNA

RNA EZ-Vision™ is a non-mutagenic fluorescent dye for staining RNA bands resolved on denaturing agarose gels containing formaldehyde. Supplied in a 1.5X loading buffer, RNA EZ-Vision™ dye stains RNA during the denaturation immediately prior to gel loading. The dye comigrates with the RNA samples during electrophoresis and provides immediate band visualization upon illumination with UV light. No post run staining or destaining is needed. The loading buffer includes formamide and bromophenol blue tracking dye.
  • Ideal for environments needing to reduce use of ethidium bromide
  • Recommended for denaturing agarose gels with formaldehyde
  • Sensitive to 150 ng of RNA
  • Compatible with downstream applications including western blots

RNA Staining with RNA EZ-Vision on a 2% Agarose Gel

RNA-Ez-Vision staining
Total RNA was purified from K562 cells with Ribozol™ RNA Extraction Reagent. Samples were denatured at 65°C for 10 minutes in RNA EZ-Vision™ loading buffer containing either 0.003% ethidium bromide or RNA EZ-Vision™ dye. The denatured RNA was loaded onto a 2% formaldehyde denaturing agarose gel (Agarose I™, Code: 0710) and resolved for 1.5 hours at 5.1 V/cm. Bands were visualized by UV illumination and image capture was performed with a Syngene GBox-HR Gel Doc System with a SYBR® Green filter.

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