Gold-N-Gel RNA Stain

Non-mutagenic, in-gel fluorescent stain for RNA visualization in denaturing agarose gels

Gold-N-Gel RNA Stain
N734
  • Safer than Ethidium Bromide
  • Immediate band visualization with UV illumination - no post-run staining or destaining
  • Easy-to-use: Simply add to melted agarose prior to casting

Supplied as a 200X concentrate, Gold-N-Gel™ RNA Stain is a non-mutagenic, fluorescent in-gel stain for immediate visualization of RNA bands on denaturing agarose gels. Gold-N-Gel™ RNA Stain is compatible with most standard protocols for denaturing electrophoresis of RNA fragments. Bands are immediately visible upon illumination with UV light. A standard Ethidium Bromide filter can be used for gel documentation.

Comparison of Gold-N-Gel™ RNA Stain to Ethidium Bromide Staining

Gold-N-Gel
Figure 1. Gold-N-Gel™ RNA staining compared to Ethidium Bromide staining (without destaining washes). Data provided by Jeff Coller, Ph.D., Case Western Reserve University, Cleveland, Ohio. Total RNA was denatured in loading buffer at 65°C for 10 minutes and loaded onto a 2% formaldehyde denaturing agarose gel containing 0.002% Ethidium Bromide or Gold-N-Gel™ RNA Dye. After electrophoresis the RNA was visualized on a UV transilluminator with an Ethidium Bromide filter.

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