Column Regeneration Kit

Regenerate DNA spin columns for multiple reuses
Column Regeneration Kit
N809

  • Efficient Regeneration
    • Removes all bound plasmid and PCR DNA from silica based mini-columns
    • Regenerated columns can be used for the same or different DNA
  • Extended Column Usage
    • Individual columns can be regenerated up to 5 times
  • Easy to use
    • Soak columns in Regeneration Solution for 1 hour to overnight
    • Centrifuge
    • Wash 3X with Wash Solution

Efficient Removal of Residual DNA from Silica-based Mini-Columns

Figure 1. PCR analysis of residual DNA recovery during regeneration with Column Regeneration Kit. pUC19 plasmid DNA, recovered from overnight cultures of JM109 cells that were lysed by alkaline lysis, was applied to silica-based mini-columns and eluted according to manufacturer’s instructions. Columns were subsequently regenerated for varying lengths of time with AMRESCO’s Column Regeneration Kit. Aliquots (15 µl) of eluates from each time point were analyzed by PCR amplification for recovery of residual DNA. Amplified products were applied to a 1% TAE agarose gel and run for 60 minutes at 80V. Gels were post-stained with 0.5 ug/mL Ethidium Bromide and images captured with a Syngene GBox-HR Gel Doc System. 
Lane 1: 5 µl PCR DNA Markers (Code: E854)
Lane 2: PCR amplification control of pUC19 plasmid (50 ng).
Lane 3: PCR amplification of mini column eluate DNA.
Lane 4: PCR amplification of eluate DNA after a 60 minute soak in H2O.
Lane 5: PCR amplification of eluate DNA after a 15 minute treatment with Regeneration Solution.
Lane 6: PCR amplification of eluate DNA after a 30 minute treatment with Regeneration Solution.
Lane 7: PCR amplification of eluate DNA after a 45 minute treatment with Regeneration Solution.
Lane 8: PCR amplification of eluate DNA after a 60 minute treatment with Regeneration Solution.

Excellent Recovery of Plasmid DNA after Purification on Regenerated Columns

N809
Figure 2. Overnight cultures of DH5α cells containing pET22b(+) plasmid were lysed by alkaline lysis and applied to silica miniprep columns that were not regenerated, regenerated only one time, or regenerated 5 times. Plasmid DNA was eluted with 100µL of 10mM Tris-HCL, pH8.5 and DNA yields were determined by absorbance at 260nm.

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