Life Science Research Products & Biochemicals
ISO 9001 & ISO 13485 Certified

Products

Cytoplasmic & Nuclear Protein Enrichment Kit

Cytoplasmic & Nuclear Protein Enrichment Kit
Code # M330

Fractionate cytoplasmic and nuclear proteins from cultured mammalian cells

Minimal cross contamination
- Distinct nuclear and cytoplasmic protein isolates
Flexible
- For adherent and non-adherent cultured cells
- Scalable protocol for variable number of cells
Easy and Fast
- Kit includes 3 buffers
- Protocol takes less than 1.5 hours
Compatible with downstream applications including Western blotting

Binding of HDAC-1 nuclear specific antibody to nuclear fraction after fraction with AMRESCO Cytoplasmic & Nuclear Protein Enrichment Kit

$Binding of HDAC-1 nuclear-specific antibody to nuclear fraction of AMRESCO Cytoplasmic & Nuclear Protein Enrichment Kit$

Figure 1. The nuclear specific antibody HDAC-1 demonstrates lack of cross contamination between nucleus and cytoplasm after fraction with the AMRESCO Cytoplasmic & Nuclear Protein Enrichment kit. (1) Cytoplasmic (15ug) and (2) nuclear (12.75ug) proteins isolated from K562 cells with AMRESCO’s Cytoplasmic/Nuclear Fractionation Kit were resolved on a 12.5% Fluorescent Sprint NEXT GEL® (M318). Resolution of protein bands was viewed in the gel with a UV transilluminator (A) prior to transfer to PVDF membranes (E578). Immuno-blotting by the HDAC-1 (nuclear-specific) antibody (B) was detected using Visiglo™ HRP Chemiluminescent Substrate Kit (N218).

Distribution of NF-κB in unstimulated K562 cells after fractionation by the AMRESCO Cytoplasmic & Nuclear Protein Enrichment Kit

Figure 2. Distribution of NF-κB in unstimulated K562 cells. Proteins isolated from K562 cells using AMRESCO’s Cytoplasmic/Nuclear Fractionation Kit (Cytoplasmic and Nuclear) and Total Cell Lysis Buffer (Total). Proteins (10ug) were loaded onto a 12.5% ™ NEXT GEL® (M318), transferred to PVDF membrane (E578), immunoblotted with NF-κB p65 (Rel A) antibody and visualized with AMRESCO’s VisiGlo™ HRP Plus Chemiluminescent Substrate Kit (N219-Kit) .