Fluorescent SPRINT NEXT GEL®
A convenient, ready-to-pour SDS-PAGE solution containing a fluorescent protein stain for immediate band visualization. Gels can be cast, run and visualized in under an hour.
Fluorescent SPRINT NEXT GEL™
Code: M317
(10% Gel)
Code: M318
(12.5% Gel)
- Pour and polymerize mini-gels in 15 minutes - no stacking gel needed
- Run gel in less than 30 minutes
- Visualize bands in less than 5 minutes on standard UV transilluminators - no staining or destaining needed!
- Sensitivity similar to Coomassie Blue®
- Compatible with downstream applications
Immediate band visualization with Fluorescent SPRINT NEXT GEL®
Total E. coli lysate was resolved on a 12.5% Fluorescent SPRINT NEXT GEL® run at 300 volts for 30 minutes. Bands were visualized immediately after the run by exposure to UV light for 3 minutes. Lane A: Total E. Coli lysate (10 µg). Lane B: AMRESCO Mid/Low Range Protein Molecular Weight Markers (Code:J450).Quick & Convenient Pouring, Running and Visualization
Easy gel casting:
Fluorescent SPRINT NEXT GEL® is provided as a ready-to-pour solution that offers speed and convenience for SDS-PAGE electrophoresis at substantially lower cost than pre-cast gels. The patent-pending proprietary chemistry of NEXT GEL® eliminates the need for stacking gels - the resolving gel is ready for loading and running immediately after polymerization, about 15 minutes. Simply add APS/TEMED to the gel solution and pour into the assembled casting cassette. Solutions are available at 10.0% and 12.5% acrylamide concentrations. The propriatary running buffer, included with NEXT GEL® acrylamide solutions, is essential for running of NEXT GELs.
Immediate Band Visualization:
Fluorescent SPRINT NEXT GEL® solutions include a fluorescent protein stain that provides immediate band visualization after electrophoresis. Additional staining, destaining or washing steps are not needed. Bands are visable with a 3-5 minute exposure on a UV transilluminator. Staining sensitivity is similar to Coomassie® Blue.
Compatibility:
Fluorescent SPRINT NEXT GEL® is compatible with downstream applications including Western blotting and 2-D electrophoresis.
Fluorescent SPRINT NEXT GEL® is provided as a ready-to-pour solution that offers speed and convenience for SDS-PAGE electrophoresis at substantially lower cost than pre-cast gels. The patent-pending proprietary chemistry of NEXT GEL® eliminates the need for stacking gels - the resolving gel is ready for loading and running immediately after polymerization, about 15 minutes. Simply add APS/TEMED to the gel solution and pour into the assembled casting cassette. Solutions are available at 10.0% and 12.5% acrylamide concentrations. The propriatary running buffer, included with NEXT GEL® acrylamide solutions, is essential for running of NEXT GELs.
Immediate Band Visualization:
Fluorescent SPRINT NEXT GEL® solutions include a fluorescent protein stain that provides immediate band visualization after electrophoresis. Additional staining, destaining or washing steps are not needed. Bands are visable with a 3-5 minute exposure on a UV transilluminator. Staining sensitivity is similar to Coomassie® Blue.
Compatibility:
Fluorescent SPRINT NEXT GEL® is compatible with downstream applications including Western blotting and 2-D electrophoresis.
Each Fluorescent SPRINT NEXT GEL® includes:
Not included:
- Ready-to-use 1X acrylamide/bis-acrylamide gel solution
- Available at 10.0% or 12.5% concentration
- Includes fluorescent protein stain
- Stable at room temperature 1 year
- NEXT GEL Running Buffer, 20X solution
Not included:
- Ammonium Persulfate, Code: 0486; also available in convenient tablet form, Code: K833
- TEMED, Code: 0761
CONTACT US
Phone
Toll-Free: 800.829.2805
(U.S. & Canada)
Local: 440.349.1199
Bulk Fine Chemicals:
Toll-Free: 800.829.2802
Technical Support
Toll-free: 800.610.2789
FAX
Local FAX: 440.349.3255
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Mailing Address
AMRESCO Inc.
6681 Cochran Road
Solon, OH 44139 USA