Agarose I
All-purpose, High-purity Agarose
Agarose I™
0710
- Exceptional Band Resolution & Clarity
- Nuclease & Protease-free
Agarose I™ is a standard melting/gelling agarose, suitable for routine nucleic acid analytical and preparative applications. Agarose I™ has a low EEO for shorter electrophoretic runs without loss of resolution. It is ideal for a variety of downstream applications including cloning, sequencing and Northern or Southern analysis.
Recommended Agarose I™ concentrations for optimal resolution
|
GEL (%) |
OPTIMAL SEPARATION RANGE (bp) |
RECOMMENDED BUFFER |
| 0.8 | 800 – 22,000 | TAE |
| 1.0 | 500 – 10,000 | TAE/TBE |
| 1.2 | 400 – 7,000 | TAE/TBE |
| 2.0 | 250 – 5,000 | TBE |
Figure 1. Superior DNA resolution on AMRESCO’s Agarose I™. Data courtesy of Dr. Mary Cismowski, Northeastern Ohio Universities College of Medicine, Rootstown, OH, USA. A 1% agarose/TAE gel containing 0.5 μg/ml Ethidium Bromide was prepared with Agarose I™. HincII digested pcDNA3.1-HisC were applied and resolved at 100 V for 55 minutes.
Lane 1: 100 bp ladder
Lane 2: 0.5 μg HincII digested pcDNA3.1
Lane 3: 0.2 μg HincII digested pcDNA3.1
Lane 4: 0.1 μg HincII digested pcDNA3.1
Lane 5: 0.05 μg HincII digested pcDNA3.1
Lane 6: 1 kb ladder
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AMRESCO LLC
6681 Cochran Road
Solon, OH 44139 USA