Non-mutagenic, environmentally friendly alternative to Ethidium Bromide
EZ-Vision® One, Two and Three.
Left image: 1% TAE agarose gel showing the fluorescence of AMRESCO’s 1kb Ladder (K180-250μl) stained with EZVision® One (lane 1), EZ-Vision® Two (lane 2) and EZ-Vision® Three (lane3) captured with Syngene GBox-HR Gel Doc System
using SP filter selection. Right image: Digital camera photograph of the same gel as left image, showing the colors and migration position of the one fast migrating tracking dye of EZ-Vision® One (lane 1), the two tracking dyes of EZ-Vision® Two (lane2), and the three tracking dyes of EZ-Vision® Three (lane 3).
EZ-Vision® Safety Testing
Figure 2. EZ-Vision® mutagenicity was determined by Ames testing of S. typhimurium
with and without metabolic activation with an S-9 activation system. Sample concentrations tested did not produce a two-fold increase in the number revertants and did not meet the criteria for a potential mutagen.
EZ-Vision® Environmental Toxicity Testing.
EZ-Vision® environmental hazard testing was determined by the CCR Title 22 Fathead Minnow Hazardous Waste Screen Bioassay. Both EZ-Vision® Two and EZ-Vision® Three were determined non-hazardous with LC50 > 750 mg/l. Additional information.
Relative migration of prestained samples of dsDNA in 1% agarose gel. The data shows that EZ-Vision® has much less effect on DNA migration than Ethidium Bromide or Competitor 2 Green Dye. Samples stained with EZ-Vision® are not impeded and migrate similar to dsDNA that is stained post electrophoresis.
Figure 4. EZ-Vision® fluorescent signal degradation in agarose gels with U.V. exposure over time.
Sample DNA was stained with EZ-Vision® or Competitor 1 Green Dye and electrophoresed on 1% agarose gels. Following electrophoresis, gels were exposed continuously to u.v. light, and the fluorescent signal quantified at 10 minute intervals using SyneGne GBox-HR Gel Doc System, presented as % of original signal. The data demonstrates that DNA stained with EZ-Vision® retains the fluorescence approximately twice as long as Competitor I.