N218
VISIGLO™ HRP CHEMILUMINESCENT SUBSTRATE KIT

Grade:

CAS Number:
N/A
Synonym(s):
chemiluminescent substrate, ECL
Molecular Weight:
N/A
Molecular Formula:
N/A
Storage Condition:
COLD
Risk Statements:
N/A
Safety Statements:
N/A
Hazard Code:
None
UN Number:
NONE
Code
Size
Price
Quantity
N218-SAMPLE-KIT

KIT

$17.30

N218-S-KIT

KIT

$89.43

N218-KIT

KIT

$127.70

Overview

Luminol-based substrate for rapid detection of peroxidase labeled conjugates.

  • Picogram range sensitivity
  • Allows for multiple stripping and reprobing

How to Use

Supplied as kit with equivalent volumes Substrate A and Substrate B for the detection of ~2400 cm2 of membrane. Mix directly before use and apply to blot.

Click on the Features tab below to learn more about VisiGlo™ HRP Chemiluminescent Substrates.


Material Safety Data Sheet
Certificate of Analysis
Directions for Use

Detailed Description

VisiGlo™ HRP and VisiGlo PLUS™ HRP are luminol-based chemiluminescent substrates designed for the rapid and sensitive detection of peroxidase labeled-conjugates. They offer increased sensitivity compared to chromogenic substrates in both blotting and ELISA applications. VisiGlo™ HRP and VisiGlo PLUS™ HRP can be detected using X-ray film and chemiluminescent imagers in blotting applications (see Comparison Chart below) or using a luminometer for ELISA. VisiGlo PLUS™ HRP is ideal for those situations where the protein of interest is expressed in low abundance. Strong signal is achieved with the use of reduced amounts of precious target and antibodies.

Sensitivity of VisiGlo™ HRP. Serial dilutions of mouse (6.1-0.024 pg) IgG were run on SDS-PAGE and transferred to membrane. Blots were developed with VisiGlo™ HRP and exposed to X-ray film for 10 minutes.

Comparison of VisiGlo™ Plus and ECL™ chemiluminescent substrate sensitivity. Serial dilutions of Mouse IgG (1 ng – 31 pg) were separated by SDS-PAGE and transferred to PVDF membranes. Protein detection was performed using HRP-labeled anti-mouse antibody and each respective substrate. Film was exposed for 10 minutes and analyzed for sensitivity and signal to noise ratios. A) VisiGLO PLUS™ HRP, B) ECL™ Plus, C) ECL Advance™.