M329
TOTAL PROTEIN CELL LYSIS BUFFER

Grade:PROTEOMICS GRADE

CAS Number:
N/A
Synonym(s):
None
Molecular Weight:
N/A
Molecular Formula:
N/A
Storage Condition:
COLD
Risk Statements:
36/37/38-42/43
Safety Statements:
36/37/39
Hazard Code:
irritant sensitizer
UN Number:
NONE
Code
Size
Price
Quantity
M329-10ML

10ML

$90.91

Overview

Lysis buffer for extraction of total cell protein.

  • Easy-to-Use
  • Non-ionic detergent reduces denaturation

How to Use

Resuspend cells in Total Protein Cell Lysis Buffer and vortex vigoursly.  Incubate the cell suspension at 4 ºC with shaking for 30 minutes.  Vortex vigoursly for 30 seconds and pellet cellular debris.  The supernatant contains the total protein lysate.

Click on the Features tab to learn more about Total Protein Cell Lysis Buffer


Material Safety Data Sheet
Certificate of Analysis
Directions for Use

Total Protein Cell Lysis Buffer

Total Protein Cell Lysis Buffer is a non-ionic detergent lysis buffer that enables efficient solubilization of the plasma and intracellular membranes, breaks weak intermolecular bonds, and solubilizes most proteins.  Total Protein Cell Lysis Buffer reduces protein denaturation, protein complex disruption and loss of enzymatic activity since it contains less detergent than a typical RIPA lysis buffer.

Total protein from K562 cells probed for HSF-1 expression

Total protein was  isolated from K562 cells using AMRESCO’s Total Protein Cell Lysis Buffer (M329) and  then resolved on a 12.5% Fluorescent SPRINT NEXT GEL® for 30 minutes at 300 V. The protein was visualized by UV transillumination (lane 1) and then transferred to PVDF. RapidBlock™ Solution (M325) was used for blocking and as the antibody diluent with HSF-1 antibody (lane 2). The blot was developed using VisiGlo Plus™ HRP Chemiluminescent Substrate Kit (N219).

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pH @25C
7.2 - 7.8
Protease (P/F)
NONE
Titration (mM)
8 - 12