Hydrophobic membranes for Western transfer, protein binding assays and membrane bound protein sequencing.
TotalBLOT PVDF membrane is a naturally hydrophobic polyvinylidene fluoride membrane that is well suited for Western transfers, protein binding assays and membrane bound protein sequencing. Our PVDF membrane, a pure white microporous solid phase support, exhibits strong hydrophobic interactions with a wide range of proteins. Immobilized proteins can be directly visualized with all common staining reagents, including Coomassie Blue, Amido Black, and Ponceau S. TotalBLOT PVDF membranes also allow sensitive immuno-detection in the nanogram to picogram levels.
Our membranes are resistant to a wide range of chemical solvents and will not shrink or disfigure during destaining in methanol. In addition, resistance to trichloroacetic acid and triethylamine allow direct insertion of the membrane into amino acid analyzers and gas phase protein sequencing systems.