N676
SERAFREE™ DMEM CRYOPRESERVATION MEDIA

Grade:TISSUE CULTURE GRADE

CAS Number:
N/A
Synonym(s):
None
Molecular Weight:
N/A
Molecular Formula:
N/A
Storage Condition:
COLD
Risk Statements:
36/37/38
Safety Statements:
36/37/39
Hazard Code:
irritant
UN Number:
NONE
Code
Size
Price
Quantity
N676-50ML

50ML

$119.43

N676-6X5ML

6X5ML

$119.43

Overview

  • Ready-to-use, animal-free RPMI based media
  • Optimizes cell growth and cell viability after thawing
  • Reduces potential for transmission of infectious diseases

Detailed Description

Serum-free RPMI based cryopreservation media for adherent and suspension cultured cells. Sterile and endotoxin tested. Suitable for use in environments that prohibit the use of animal-derived products.

Viability of post-thaw cultures of K562 cells.

Figure 1. Cells frozen in AMRESCO SeraFree™ Cryoprotection Media () exhibited higher recovery than cells frozen in traditional RPMI freezing media containing DMSO and serum (). Viability of cells recovering from cryopreservation in AMRESCO SeraFree™ Cryoprotection Media was comparable or better than that of cells in Competitors 1 () and 2 () serum-free freezing media. Viability was determined by standard trypan-blue exclusion assay.

Performance of AMRESCO SeraFree™ Cryopreservation Media rival serum-containing media.

HeLa cells (1x106) were frozen in AMRESCO’s DMEM () or RPMI () SeraFree™ media, serum-containing freezing media () and a competitor’s serum-free freezing media (). Thawed cells were counted using standard trypan blue exclusion assay.

Material Safety Data Sheet
Certificate of Analysis
Directions for Use
Endotoxins (EU/mL)
< 1
Milliflex (P/F)
PASS
Osmolality (mOsm)
2000 - 2200
pH @25C
7.2 - 8.0