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ISO 9001 & ISO 13485 Certified

N806
READY PCR MIX, 2X

Grade:BIOTECHNOLOGY GRADE

CAS Number:
NA
Synonym(s):
PCR Master Mix PCR to Gel
Molecular Weight:
NA
Molecular Formula:
NA
Storage Condition:
FRZ
Risk Statements:
36/37/38
Safety Statements:
36/37/38
Hazard Code:
irritant
UN Number:
NA
Code
Size
Price
Quantity
N806-1.25ML

1.25ML

$50.00

N806-2X1.25ML

2X1.25ML

$90.91

Overview

Ready PCR Mix, 2X is the only PCR master mix that takes you from PCR to Gel to Visualization in one easy step.

  • Direct to Gel Loading
  • Immediate Visualization
  • Safe to Use

Detailed Description

Ready PCR Mix, 2X offers a single-step procedure for performing PCR reactions followed by analysis on agarose gels without the addition of loading buffer.  All components for assembly and performance of PCR reactions as well as loading and visualization of PCR products on agarose gels are included.  The user supplies primer and template DNA.

Combine primers, DNA template, nuclease-free water, and Ready PCR Mix, 2X in a PCR tube.  After product amplication, directly load the PCR reactions to a gel and separate.  Once electrophoresis is complete, visualize the DNA product using a standard UV illuminator.

Click on the Features tab to learn more about Ready PCR Mix, 2X


Material Safety Data Sheet
Certificate of Analysis
Directions for Use

Ready PCR Mix, 2X

Ready PCR Mix is supplied as a 2X mixture of reaction buffer, Extender™ DNA Polymerase Blend, dNTPs, electrophoresis tracking dye, and a non-mutagenic EZ-Vision® visualization dye. Once amplification is complete, the PCR reaction can be directly loaded and separated on an agarose gel using the magenta-colored tracking dye (migrates at approximately 10 bp on a 1% gel) to monitor the migration.  After electrophoresis the PCR products are immediately visualized with standard UV illumination without additional post-run staining and destaining steps.  Extender™ PCR-to-Gel Master Mix, 2X (N867) contains the same formulation as Ready PCR Mix, 2X without the fluorescent DNA dye.

Efficient amplification of purified DNA with Ready PCR Mix, 2X



Single copy targets were amplified from 50 ng of pUC19 plasmid (A) or S. aureus genomic DNA (B) for 35 cycles.  Aliquots (10 µL) of each PCR reaction was directly loaded onto a 1% agarose gel and visualized with a Syngene HR gel doc system.

Direct Colony Screening with AMRESCO PCR Master Mixes

Ten distinct colonies were suspended and separated into PCR tubes.  The PCR reaction mix (5 µL) was plated on gridded LB plates with kanamycin for 37 ºC overnight growth.  After 30 PCR cycles, products were screened on a 1% agarose gel.  Colonies 4, 8, 9, 10 contained the desired sequences. Courtesy of Dr. Irene Lee, Case Western Reserve University, Cleveland, OH.

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Abs.@515-525nm (50uL/950uL Water)
0.36 - 0.38
DNase (P/F)
NONE
PCR
PASS