N580
RIBOZOL™ RNA EXTRACTION REAGENT

Grade:BIOTECHNOLOGY GRADE

CAS Number:
N/A
Synonym(s):
None
Molecular Weight:
N/A
Molecular Formula:
N/A
Storage Condition:
ROOM TEMPERATURE
Risk Statements:
26/27/28-36/37/38-34
Safety Statements:
36/37/39
Hazard Code:
irritant corrosive highly-toxic
UN Number:
2821
Code
Size
Price
Quantity
N580-30ML

30ML

$47.73

N580-100ML

100ML

$107.95

N580-200ML

200ML

$198.86

Overview

  • Isolate intact total RNA from even the most difficult of cells and tissue types
  • Extract total RNA in less than one hour
  • Achieve consistent results and excellent recovery of small RNAs
  • Compatible with a variety of applications including Northern analysis, dot blots, cloning, in vitro translation, poly (A+) selectiion and RT-PCR

Detailed Description

Ribozol™ RNA Extraction Reagent is a single phase phenol solution optimized for the isolation of total RNA from a variety of cell and tissue types. Homogenization or disruption directly in Ribozol™ RNA Extraction Reagent inhibits RNase activity to substantially minimize degradation of all classes of RNA. The simple and effective procedure for isolation in Ribozol™ RNA Extraction Reagent includes homogenization, phase separation, RNA precipitation, RNA wash and solubilization.

Click on the features tab to learn more about RiboZol™ RNA Extraction Reagent 


Material Safety Data Sheet
Certificate of Analysis
Directions for Use

RiboZol™ RNA Extraction Reagent

RiboZol™ is a ready-to-use, single phase RNA extraction reagent that produces high yields of total RNA in less than one hour. RiboZol™ yields excellent results from even the most difficult of cells and tissues and is compatible with a variety of downstream applications including: Northern analysis, dot blots, cloning, in vitro translation of  Poly (A+) selected RNA, and RT-PCR.

Agarose gel electrophoresis of RiboZol extracted RNA


Total RNA was extracted from K562 cells (1x106 cells) with RiboZol™ RNA Extraction Reagent. RNA pellets were resuspended in RiboReserve™ RNA Storage Buffer. Samples were electrophoresed on a 1.2% Agarose I formaldehyde gel. Lane 1: RiboReady™ 1 Kb RNA Ladder; Lanes 2 & 3: RiboZol™ extracted RNA samples.

High yields of total RNA with RiboZol™ RNA Extraction Reagent


RiboZol™ extracted RNA yields from 2 different cell lines. Data provided by Eunmi Ho, Catholic University, Korea. RNA was extracted from HeLa cell cultures (purple bars) 1x106 cells and from CaSki cultures (magenta bars) 1x106 cells according to standard procedures. Total RNA yields were determined by OD260

Excellent Purity

Purity of Ribozol RNA

Purity of RiboZol™ extracted RNA from 2 different cell lines. Data provided by Eunmi Ho, Catholic University, Korea. RNA was extracted from HeLa cell cultures (purple bars) 1x106 cells and from CaSki cultures (magenta bars) 1x106 cells according to provided procedures. RNA purity was determined by the OD260/280 ratio.

Compatible with downstream applications

cDNA synthesis/PCR from Ribozol extracted RNA

cDNA synthesis/PCR from RiboZol™ extracted RNA. Data provided by Beth Sullivan, PhD. Duke University Medical Center, Durham, NC. cDNA synthesis: mRNA was extracted from GM08854-mouse/ Human Chr21 hybrid cells with RiboZol™ or with a competitor’s reagent. 2 μg samples were added to SuperScript®III First Strand Synthesis System (Invitrogen) as templates for reverse transcription. PCR reactions: 0.5 μl of each cDNA was added to PCR reactions containing primers to mouse beta actin. Samples were removed after 35 cycles and loaded onto 2% agarose gels.
Lane 1: 100 bp ladder;
Lane 2: water;
Lane 3: RiboZol™ RNA - RT;
Lane 4: RiboZol™ RNA + RT;
Lane 5: Competitor RNA - RT;
Lane 6: Competitor RNA + RT.

Related Products

  • RiboZol™ Plus Total RNA Purification Kit (N643) 
  • RiboReserve™ RNA Storage Buffer (N633)
  • Water, Sterile, Nuclease-Free (E476) 
Abs.@ Lambda Max
1.50 - 2.70
DNase (P/F)
NONE
Electrophoresis (P/F)
PASS
Lambda Max (nm)
530 - 534
Percent OD260/280 (%)
95
Percent Yield (%)
80
pH @25C
3.8 - 4.2
RNase (P/F)
NONE
Specific Gravity @20C
1.0900 - 1.1200
Water Content (KF) (%)
36 - 42