M328
MITOCHONDRIAL PROTEIN ISOLATION BUFFER

Grade:PROTEOMICS GRADE

CAS Number:
N/A
Synonym(s):
None
Molecular Weight:
N/A
Molecular Formula:
N/A
Storage Condition:
COLD
Risk Statements:
N/A
Safety Statements:
36/37/39
Hazard Code:
None
UN Number:
NONE
Code
Size
Price
Quantity
M328-30ML

30ML

$96.59

Overview

Mitochondrial protein isolation buffer for enrichment of mitochondrial proteins.

  • Entire extraction procedure takes less than an hour
  • Single component
  • Compatible with Western blotting & ELISA assays

How to Use

Cells are lysed with a syringe and then centrifuged to separate the cytosolic fraction from an enriched mitochondrial fraction.  The collected fractions may be used in downstream applications, including Western blotting and ELISA assays. 

Click on the Features tab to learn more about Mitochondrial Protein Isolation Buffer


Material Safety Data Sheet
Certificate of Analysis
Directions for Use

Mitochondrial Protein Isolation Buffer

AMRESCO’s Mitochondrial Protein Isolation Buffer is a convenient one-buffer solution for extraction of protein from mitochondrial fractions derived from cultured mammalian cells. The simple, scalable extraction procedure requires less than an hour and involves no toxic chemicals or ultracentrifugation steps. Following cell lysis, the cytostolic fraction is separated by centrifugation from an enriched mitochondrial fraction. This separation allows the less complex set of mitochondrial proteins to be analyzed in a single sample and increases the likelihood of detecting low-abundance proteins. The mitochondrial protein fraction can be used to perform Western blotting, ELISA or other assays.

Detection of EndoG in mitochondrial protein isolated from K562 cells

Mitochondrial Protein Isolation Buffer (M328) was used to isolate mitochondrial (lanes A1, A3, B1, B3) and cytoplasmic (lanes A2, A4, B2, B4) protein fractions from K562 cells.  The proteins were resolved on a 12.5% Fluorescent SPRINT NEXT GEL® (M318) for 30 minutes at 300 volts, then visualized by UV transillumination (Figure A).  The proteins were transferred to PVDF and then immunoblotted for β-tubulin (lanes B1, B2) and EndoG (lanes B3, B4) using RapidBlock™ Solution (M325) (Figure B).  Bands were detected using VisiGlo Plus™ HRP Chemiluminescent Substrate Kit (N219).

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pH @25C
7.7 - 8.1
Protease (P/F)
NONE
Titration (mM)
8 - 12