Now available in convenient, ready-to-use dropper bottles!
Dilute Gold-N-Gel™ RNA Stain, 200X to 1X in molten MOPS-agarose. Cast the gel, load heat-denatured RNA samples, and separate the RNA. Visualize your bands.
Supplied as a 200X concentrate, Gold-N-Gel™ RNA Stain is a non-mutagenic, fluorescent in-gel stain for immediate visualization of RNA bands on denaturing agarose gels. Gold-N-Gel™ RNA Stain is compatible with most standard protocols for denaturing electrophoresis of RNA fragments. Bands are immediately visible upon illumination with UV light. A standard Ethidium Bromide filter can be used for gel documentation.
Figure 1. Gold-N-Gel™ RNA staining compared to Ethidium Bromide staining (without destaining washes). Data provided by Jeff Coller, Ph.D., Case Western Reserve University, Cleveland, Ohio. Total RNA was denatured in loading buffer at 65°C for 10 minutes and loaded onto a 2% formaldehyde denaturing agarose gel containing 0.002% Ethidium Bromide or Gold-N-Gel™ RNA Dye. After electrophoresis the RNA was visualized on a UV transilluminator with an Ethidium Bromide filter.
Omnilab Srl.
Buenos Aires
Argentina
Tel: 54-11-4553-1100
Fax: 54-11-4555-1584
E-mail: info@omnilab.com.ar
Website: www.omnilab.com.ar