AMRESCO’s Fluorescent DNA Quantitation Kit uses the fluorescent bisBenzimide H33258 dye to bind to AT sequences of the minor groove of double-stranded DNA. In the assay, the sample is excited at 360 nm and when the dye binds to dsDNA an emission spectrum at 460 nm is produced. AMRESCO’s Fluorescent DNA Quantitation Kit can be used to quantify dsDNA in the low nanogram per mL (10 ng/mL) range, up to 5 microgram per mL (5 µg/mL). This assay can also tolerate RNA or protein contamination that may be present in crude extracts.
Fluorescent DNA Assay Buffer, 10X, 200 ml
Fluorescent DNA Dye, 250 µl
Fluorescent DNA Standard, 1 ml
Contains sufficient reagents for 750 x 2 ml reactions.