M318
FLUORESCENT SPRINT NEXT GEL® 12.5%

Grade:

CAS Number:
N/A
Synonym(s):
None
Molecular Weight:
N/A
Molecular Formula:
N/A
Storage Condition:
ROOM TEMPERATURE
Risk Statements:
N/A
Safety Statements:
N/A
Hazard Code:
None
UN Number:
NONE
Code
Size
Price
Quantity
M318-TRIAL-KIT

KIT

$18.50

M318-KIT-100ML

100ML

$57.39

M318-KIT-500ML

500ML

$265.57

  

Overview

A ready-to-pour acrylamide solution for rapid SDS-PAGE resolution of proteins. The acrylamide mix includes a fluorescent stain for in-gel staining of proteins that provides immediate visualization of resolved bands. Recommended separation range for 12.5% solution: 3.5kDa - 100kDa.

  • No stacking gel required
  • Fluorescent dye in acrylamide binds to proteins for direct visualization by UV light
  • Eliminates post-staining and destaining steps
  • For the separation of proteins from 3.5 kDa - 100 kDa

How to Use

Supplied as kit for the preparation of either 10 or 50 mini gels. Each kit contains one bottle Fluorescent Sprint NEXT GEL® acrylamide solution and 2 bottles of 20X NEXT GEL® Running Buffer.

Click on the Features tab below for a detailed description of Fluorescent Sprint NEXT GEL®


Material Safety Data Sheet
Certificate of Analysis
Directions for Use

Detailed Description

  • Pour and polymerize mini-gels in 15 minutes - no stacking gel needed
  • Run gel in less than 30 minutes
  • Visualize bands in less than 5 minutes on standard UV transilluminators - no staining or destaining needed!
  • Sensitivity similar to Coomassie Blue®
  • Compatible with downstream applications

Fluorescent SPRINT NEXT GEL

Immediate band visualization with Fluorescent SPRINT NEXT GEL®

Total E. coli lysate was resolved on a 12.5% Fluorescent SPRINT NEXT GEL® run at 300 volts for 30 minutes. Bands were visualized immediately after the run by exposure to UV light for 3 minutes. Lane A: Total E. Coli lysate (10 µg). Lane B: AMRESCO Mid/Low Range Protein Molecular Weight Markers (Code:J450).

Quick & Convenient Pouring, Running and Visualization

Fluorescent SPRINT NEXT GEL

Easy gel casting:
Fluorescent SPRINT NEXT GEL® is provided as a ready-to-pour solution that offers speed and convenience for SDS-PAGE electrophoresis at substantially lower cost than pre-cast gels. The patent-pending proprietary chemistry of NEXT GEL® eliminates the need for stacking gels - the resolving gel is ready for loading and running immediately after polymerization, about 15 minutes. Simply add APS/TEMED to the gel solution and pour into the assembled casting cassette. Solutions are available at 10.0% and 12.5% acrylamide concentrations. The propriatary running buffer, included with NEXT GEL® acrylamide solutions, is essential for running of NEXT GELs.

Immediate Band Visualization:
Fluorescent SPRINT NEXT GEL® solutions include a fluorescent protein stain that provides immediate band visualization after electrophoresis. Additional staining, destaining or washing steps are not needed. Bands are visable with a 3-5 minute exposure on a UV transilluminator. Staining sensitivity is similar to Coomassie® Blue. 

Compatibility:
Fluorescent SPRINT NEXT GEL® is compatible with downstream applications including Western blotting and 2-D electrophoresis.

Each Fluorescent SPRINT NEXT GEL® includes:

  • Ready-to-use 1X acrylamide/bis-acrylamide gel solution
    • Available at 10.0% or 12.5% concentration
    • Includes fluorescent protein stain
    • Stable at room temperature 1 year
  • NEXT GEL Running Buffer, 20X solution

Not included: