N650
EZ-VISION™ TWO, DNA DYE AS LOADING BUFFER

Grade:BIOTECHNOLOGY GRADE

CAS Number:
N/A
Synonym(s):
DNA loading buffer, DNA loading dye
Molecular Weight:
N/A
Molecular Formula:
N/A
Storage Condition:
COLD
Risk Statements:
36/37/38
Safety Statements:
36/37/39
Hazard Code:
irritant
UN Number:
NONE
Code
Size
Price
Quantity
N650-Q-SAMPLE

SAMPLE

$20.80

N650-KIT

KIT

$115.40

EZ-Vision™ Two is a non-mutagenic, environmentally friendly alternative to ethidium bromide for instant visualization of  DNA bands after electrophoresis in agarose gels.  Supplied in AMRESCO’s 6X DNA Loading Buffer, EZ-Vision™ Two complexes with the sample DNA and co-migrates with it during electrophoresis. Results can be visualized immediately after the run by placing the gel on a standard UV transilluminator. Post-run staining and destaining is eliminated. It is ideal for applications needing rapid DNA band visualization and for environments requiring a safe, non-hazardous alternative to ethidium bromide. EZ-Vision™ Two contains 2 tracking dyes that migrate at 4,000 bp and 400 bp in a 1% agarose gel. Formulations also available with three tracking dyes (code N313-KIT) or one tracking dye (code N472-KIT).
Click on the Features tab below for additional information

Material Safety Data Sheet
Certificate of Analysis
Directions for Use

AMRESCO DNA Electrophoresis Products
EZ-Vision™/Agaroses/Molecular Weight Markers/Running Buffers
EZ-Vision Two DNA Dye as Loading Buffer

AMRESCO's non-mutagenic, environmentally friendly alternative to ethidium bromide

Simple, Safe and Sensitive

Easy and Convenient
  • Fluorescent DNA dye supplied in a 6X loading buffer
  • Simply add EZ-Vision™ to your samples and load your agarose gel
Instant Results
  • Visualize DNA instantly with a standard U.V. transilluminator
  • Very low background
  • Requires no post electrophoresis staining or destaining
  • Works with most existing filters for gel documentation.
  • Very broad emission spectra with peak near 450 nm
Better for you and the environment than ethidium bromide
  • Non-mutagenic
  • Non-toxic for waste disposal


Agarose Gel Electrophoresis of EZ-Vision™ One, Two and Three Stained DNA

Agarose Gel Electrophoresis of EZ-Vision One, Two and Three


EZ-Vision™ One, Two and Three.
Left image: 1% TAE agarose gel showing the fluorescence of AMRESCO’s 1kb Ladder (K180-250μl) stained with EZVision ™ and captured with a Syngene GBox-HR Gel Doc System using SP filter selection.
    Lane 1: EZVision ™ One
    Lane 2: EZ-Vision™ Two
    Lane 3: EZ-Vision™ Three  
Right image: Migration positions and colors of EZVision ™ tracking dyes. Digital photograph of the same gel as the left image.
    Lane 1: EZVision ™ One
    Lane 2: EZ-Vision™ Two
    Lane 3: EZ-Vision™ Three

EZ-Vision™ Safety Testing

EZ-Vision™ mutagenicity was determined by Ames testing of S. typhimurium with and without metabolic activation with an S-9 activation system. Sample concentrations tested did not produce a two-fold increase in the number revertants and did not meet the criteria for a potential mutagen. Additional information.
EZ-Vision Mutagencity Testing

EZ-Vision™ environmental hazard testing was determined by the CCR Title 22 Fathead Minnow Hazardous Waste Screen Bioassay. Both EZ-Vision™ Two and EZ-Vision™ Three were determined non-hazardous with LC50 > 750 mg/l. Additional information
EZ-Vision Environmental Hazard Testing

EZ-Vision™ Properties

  • Does not alter migration of DNA during electrophoresis
  • Retains fluorescent signal longer than leading competitor
  • Sensitivity similar to Ethidium Bromide at 6ng of DNA above 500 bp, and at 12ng of DNA at 50 bp.
EZ-Vision does not retard migration of DNA during electrophoresis in agarose gels




Relative migration of prestained samples of dsDNA in1% agarose gel with EZVision ™, Competitor 2 Green Dye, and Ethidium Bromide. Samples stained with EZ-Vision™ are not impeded and migrate at a rate similar to dsDNA that is stained post electrophoresis. The mobility of samples stained with ethidium bromide or competitor 2 green dye is reduced relative to unstained DNA.
EZ-Vision fluorescence decay rate relative to competitors



EZ-Vision fluorescent signal degradation in agarose gels with U.V. exposure over time. Sample DNA was stained with EZ-Vision™ or Competitor 1 Green Dye and electrophoresed on 1% agarose gels. Following electrophoresis, gels were exposed continuously to u.v. light, and the fluorescent signal quantified at 10 minute intervals using SyneGne GBox-HR Gel Doc System, presented as % of original signal. The data demonstrates that DNA stained with EZ-Vision™ retains the fluorescence approximately twice as long as Competitor I.
Abs.@595-615nm (1:500mL Water)
0.5 - 0.9
DNase (P/F)
NONE
Electrophoresis (P/F)
PASS
Protease (P/F)
NONE