N313
EZ-VISION™ THREE, DNA DYE AS LOADING BUFFER 6X

Grade:BIOTECHNOLOGY GRADE

CAS Number:
N/A
Synonym(s):
DNA loading buffer, DNA loading dye
Molecular Weight:
N/A
Molecular Formula:
N/A
Storage Condition:
COLD
Risk Statements:
36/37/38
Safety Statements:
36/37/39
Hazard Code:
irritant
UN Number:
NONE
Code
Size
Price
Quantity
N313-Q-SAMPLE

SAMPLE

$20.80

N313-KIT

KIT

$115.40

EZ-VISION™ DNA dyes provide a safe, non-toxic and non-mutagenic alternative to Ethidium Bromide for instantaneous band visualization. EZ-VISION™ is supplied as a 6X loading buffer. Simply mix with DNA sample, run on gel and immediately visualize bands on an UV transilluminator. EZ-VISION™ DNA dyes are environmentally friendly containing no hazardous chemicals that require special handling or disposal. EZ-VISION Three™ DNA Dye as Loading Buffer features an exclusive blend of 3 tracking dyes that make estimating sample migration simple and easy. The 3 dyes run at 10 bp, 400 bp, and 4000 bp on a 1% agarose gel. Formulations also available with a single tracking dye (code N472-KIT) or two tracking dyes (code N650-KIT).

Click on the features tab below for additional EZ-VISION™ information.

Material Safety Data Sheet
Certificate of Analysis
Directions for Use
EZ-Vision Three DNA Dye as Loading Buffer

AMRESCO's non-mutagenic, environmentally friendly alternative to ethidium bromide
Easy and Convenient
  • Fluorescent DNA dye supplied in a 6X loading buffer
  • Simply add EZ-Vision™ to your samples and load your agarose gel
Instant Results
  • Visualize DNA instantly with a standard U.V. transilluminator
  • Very low background
  • Requires no post electrophoresis staining or destaining
  • Works with most existing filters for gel documentation.
  • Very broad emission spectra with peak near 450 nm
Better for you and the environment than ethidium bromide
  • Non-mutagenic
  • Non-toxic for waste disposal


Agarose Gel Electrophoresis of EZ-Vision™ One, Two and Three Stained DNA

Agarose Gel Electrophoresis of EZ-Vision stained DNA Fragments


EZ-Vision™ One, Two and Three.
Left image: 1% TAE agarose gel showing the fluorescence of AMRESCO’s 1kb Ladder (K180-250μl) stained with EZVision ™ and captured with a Syngene GBox-HR Gel Doc System using SP filter selection.
Lane 1 : EZVision ™ One
Lane 2 : EZ-Vision™ Two
Lane 3 : EZ-Vision™ Three
Right image : Migration positions and colors of EZVision ™ tracking dyes. Digital photograph of the same gel as the left image.
Lane 1 : EZVision ™ One
Lane 2 : EZ-Vision™ Two
Lane 3 : EZ-Vision™ Three

EZ-Vision™ Safety Testing

EZ-Vision™ mutagenicity was determined by Ames testing of S. typhimurium with and without metabolic activation with an S-9 activation system. Sample concentrations tested did not produce a two-fold increase in the number revertants and did not meet the criteria for a potential mutagen. Additional information
EZ-Vision mutagenicity testing results.
EZ-Vision™ environmental hazard testing was determined by the CCR Title 22 Fathead Minnow Hazardous Waste Screen Bioassay. Both EZ-Vision™ Two and EZ-Vision™ Three were determined non-hazardous with LC50 > 750 mg/l.
Additional information
EZ-Vision environmental hazard testing results

EZ-Vision™ Properties

  • Does not alter migration of DNA during electrophoresis
  • Retains fluorescent signal longer than leading competitor
  • Sensitivity similar to Ethidium Bromide at 6ng of DNA above 500 bp, and at 12ng of DNA at 50 bp.
Migration of EZ-Vision stained DNA in agarose gels



Relative migration of prestained samples of dsDNA in1% agarose gel with EZVision ™, Competitor 2 Green Dye, and Ethidium Bromide. Samples stained with EZ-Vision™ are not impeded and migrate at a rate similar to dsDNA that is stained post electrophoresis. The mobility of samples stained with ethidium bromide or competitor 2 green dye is reduced relative to unstained DNA.
Decay of EZ-Vision Fluorescence Intensity in Agarose Gels


EZ-Vision fluorescent signal degradation in agarose gels with U.V. exposure over time. Sample DNA was stained with EZ-Vision™ or Competitor 1 Green Dye and electrophoresed on 1% agarose gels. Following electrophoresis, gels were exposed continuously to u.v. light, and the fluorescent signal quantified at 10 minute intervals using SyneGne GBox-HR Gel Doc System, presented as % of original signal. The data demonstrates that DNA stained with EZ-Vision™ retains the fluorescence approximately twice as long as Competitor I.
Abs.@ 525nm (1:500mL, Water)
0.4 - 0.8
Abs.@ 588-594nm (1:500mL, Water)
0.5 - 0.8
Abs.@ 635-641nm (1:500mL, Water)
0.55 - 0.9
Clarity (P/F)
PASS
DNase (P/F)
NONE
Electrophoresis (P/F)
PASS
Protease (P/F)
NONE