N472
EZ-VISION® ONE, DNA DYE AS LOADING BUFFER 6X

Grade:BIOTECHNOLOGY GRADE

CAS Number:
N/A
Synonym(s):
EZ-Vision One DNA Dye as Loading Buffer, EZ-Vision One
Molecular Weight:
N/A
Molecular Formula:
N/A
Storage Condition:
COLD
Risk Statements:
36/37/38
Safety Statements:
36/37/39
Hazard Code:
irritant
UN Number:
NONE
Code
Size
Price
Quantity
N472-Q-0.5ML

0.5ML

$23.64

N472-KIT

KIT

$131.14

EZ-Vision DNA Dye as Loading Buffer

AMRESCO’s non-mutagenic, environmentally friendly alternative to Ethidium Bromide

  

Overview

Easy and Convenient
  • Fluorescent DNA dye supplied in a 6X loading buffer

Instant Results
  • Visualize DNA instantly with a standard U.V. transilluminator
  • Very low background
  • Requires no post electrophoresis staining or destaining
  • Works with most existing filters for gel documentation.
  • Very broad emission spectra with peak near 450 nm

Better for you and the environment than ethidium bromide 
  • Non-mutagenic and non-toxic
  • Non-toxic for waste disposal
  • No hazardous shipping, storage or disposal fees

Click on the Features tab to learn more about EZ-Vision® DNA Dyes as Loading Buffer, 6X


Material Safety Data Sheet
Certificate of Analysis
Directions for Use

AMRESCO DNA Electrophoresis Products
EZ-Vision™/Agaroses/Molecular Weight Markers/Running Buffers

EZ-Vision®, DNA Dye as Loading Buffer, 6X

EZ-Vision® is a non-mutagenic fluorescent reagent that produces instant visualization of DNA bands upon UV illumination of agarose gels. EZ-Vision® is non-mutagenic and non-toxic with no hazardous shipping, handling or disposal costs. Supplied in AMRESCO’s 6X DNA Loading Buffer, EZ-Vision® forms a tight complex with the sample DNA and co-migrates with it during electrophoresis. Post-run staining and destaining is completely eliminated and results can be visualized immediately after the run by placing the gel on a standard UV transilluminator. It is ideal for applications needing rapid DNA band visualization and for environments requiring a safe, non-hazardous alternative to Ethidium Bromide.

EZ-Vision® is available in 3 versions that differ only by the tracking dyes included in the loading buffer. EZ-Vision® Three contains 3 tracking dyes that migrate at 4,000 bp, 400 bp, and 10 bp. EZ-Vision® Two contains 2 tracking dyes that migrate at 4,000 bp and 400 bp. EZ-Vision® One contains only a single fast-running tracking dye that migrates at approximately 10 bp in a 1% agarose gel.

Available with 3 different tracking dye options!

EZ-Vision Gels
EZ-Vision® One, Two and Three. Left image: 1% TAE agarose gel showing the fluorescence of AMRESCO’s 1kb Ladder (K180-250μl) stained with EZVision® One (lane 1), EZ-Vision® Two (lane 2) and EZ-Vision® Three (lane3) captured with Syngene GBox-HR Gel Doc System using SP filter selection. Right image: Digital camera photograph of the same gel as left image, showing the colors and migration position of the one fast migrating tracking dye of EZ-Vision® One (lane 1), the two tracking dyes of EZ-Vision® Two (lane2), and the three tracking dyes of EZ-Vision® Three (lane 3).

EZ-Vision® sensitivity compared to Ethidium Bromide

DNA MW Marker, 1KB Ladder (K181) was mixed with EZ-Vision® Two, DNA Dye as Loading Buffer, 6X or a loading buffer containing 0.5 µg/mL ethidium bromide.  After electrophoresis, the gel was visualized on a standard UV illuminator. 


EZ-Vision® Environmental Toxicity Testing

EZ-Vision Environmental Hazard Testing
EZ-Vision® environmental hazard testing was determined by the CCR Title 22 Fathead Minnow Hazardous Waste Screen Bioassay. Both EZ-Vision® Two and EZ-Vision® Three were determined non-hazardous with LC50 > 750 mg/l.  Additional information.

Relative migration of prestained samples of dsDNA in 1% agarose gel

EZ-Vision does not retard DNA migration in agarose gels
The data shows that EZ-Vision® has much less effect on DNA migration than Ethidium Bromide or Competitor 2 Green Dye. Samples stained with EZ-Vision® are not impeded and migrate similar to dsDNA that is stained post electrophoresis.

Related Products

  • Agarose I™ (0710)
  • Agarose SFR™ (J234)
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  • TBE, 10X Liquid Concentrate (0658)
  • TBE, Ready-Pack™, 10X (0478)
  • TAE, 25X Liquid Concentrate (0796)
  • DNA MW Marker, 100bp Ladder (K180)
  • PCR DNA Marker™ (E854)
  • DNA MW Marker, 1KB Ladder (K181)
Abs.@340nm (1:100, DI Water)
0.80 - 1.21
Abs.@521nm (1:500, DI Water)
0.30 - 0.45
Clarity (P/F)
PASS
DNase (P/F)
NONE
Electrophoresis (P/F)
PASS
Protease (P/F)
NONE