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ISO 9001 & ISO 13485 Certified

N638
EXTENDER™ DNA POLYMERASE BLEND, 500U

Grade:

CAS Number:
N/A
Synonym(s):
None
Molecular Weight:
N/A
Molecular Formula:
N/A
Storage Condition:
FROZEN
Risk Statements:
N/A
Safety Statements:
N/A
Hazard Code:
None
UN Number:
NONE
Code
Size
Price
Quantity
N638-KIT

KIT

$153.41

Overview

A high fidelity polymerase cocktail for improved length and acuracy during DNA amplification.

  • Superior yields and purity
  • Ideal for amplification of long templates
  • Excellent for hard-to-copy templates
  • Amplified products include a mixture of blunt ends and 3'dA overhangs
  • Wide tolerance for Mg+2, salt concentration, pH and template contaminants

How to Use

Chose the appropriate buffer and magnesium concentration.  Combine the buffer, magnesium, dNTPs, primers, template DNA, and 2 U of Extender™ DNA Polymerase Blend.  Amplify the DNA with an optimized PCR cycle.

Click on the Features tab to learn more about Extender™ DNA Polymerase Blend, 500U


Material Safety Data Sheet
Certificate of Analysis
Directions for Use

Extender™ DNA Polymerase Blend, 500U

Extender™ DNA Polymerase is a high fidelity, thermostable enzyme blend optimized to generate high product yields and purity.  This polymerase blen includes 3' to 5' proofreading activity for accurate template replication.  It is recommended for amplification of critical templates containing GC-rich regions, palindromes, or multiple repeats.  Extender™ DNA Polymerase exhibits wide tolerance for magnesium and salt concentrations, making it excellent for multiplex amplifications.  A mixture of blunt end and 3'dA overhang amplification products are generated.  Amplification of DNA products up to 20 kb can be achieved with the optimized buffers included in the kit.

DNA amplification by Extender™ DNA Polymerase Blend

All reactions included 0.85 µL Extender™ DNA Polymerase Blend and 3% formamide.
Lane 1: Lambda/HindIII Marker.
Lane 2: 8 kb amplification product
Lane 3: 10 kb amplification product
Lane 4: 12b amplification product
Lane 5: 15 kb amplification product
Lane 6: 20 kb amplification product
Lane 7: 30 kb amplification product
Lane 8: 40 kb amplification product
Lane 9: 48 kb lambda DNA

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