background
ISO 9001 & ISO 13485 Certified

N809
COLUMN REGENERATION KIT

Grade:

CAS Number:
NA
Synonym(s):
Column Regeneration Solution
Molecular Weight:
NA
Molecular Formula:
NA
Storage Condition:
Room Temperature
Risk Statements:
34-26/27/28
Safety Statements:
36/37/39
Hazard Code:
corrosive highly-toxic
UN Number:
1760
Code
Size
Price
Quantity
N809-KIT

KIT

$62.50

Overview

A kit that regenerates silica-based mini-columns for multiple re-use.  

  • Columns can be regenerated up to 5 times
  • Simple Protocol 

How to Use

After purification of DNA, the columns are soaked in regeneration solution for at least one hour.  Centrifuge the columns to remove the regeneration solution, wash with Regeneration Wash Buffer, and centrifuge.

Click on the Features tab to learn more about Column Regeneration Kit


Material Safety Data Sheet
Certificate of Analysis
Directions for Use

Column Regeneration Kit

AMRESCO Column Regeneration Kit removes all bound plasmid and PCR DNA from silica-based mini-columns to regenerate them for multiple re-uses. After the regeneration procedure, the column can be re-used for the same or different DNA. Columns can be regenerated up to 5 times.

  • Regenerate columns up to 5 times
  • Easy-to-Use
  • Columns can be re-used for the same or different target DNA

Efficient removal of residual DNA from silica-based mini-columns

PCR analysis of residual DNA recovery during regeneration with Column Regeneration Kit. pUC19 plasmid DNA, recovered from overnight cultures of JM109 cells lysed by alkaline lysis, was applied to silica-based mini-columns and eluted according to manufacturer’s instructions. Columns were subsequently regenerated for varying lengths of time with AMRESCO’s Column Regeneration Kit. Aliquots (15 µl) of eluates from each time point were analyzed by PCR amplification for recovery of residual DNA. Amplified products were applied to a 1% TAE agarose gel and run for 60 minutes at 7 V/cm. Gels were post-stained with 0.5 mg/mL Ethidium Bromide and images captured with a Syngene GBox-HR Gel Doc System.   

Lane 1: 5 µl PCR DNA Markers (E854)
Lane 2: PCR amplification control of pUC19 plasmid (50 ng).
Lane 3: PCR amplification of mini column eluate DNA.
Lane 4: PCR amplification of eluate DNA after a 60 minute soak in H2O.
Lane 5: PCR amplification of eluate DNA after a 15 minute treatment with Regeneration Solution.
Lane 6: PCR amplification of eluate DNA after a 30 minute treatment with Regeneration Solution.
Lane 7: PCR amplification of eluate DNA after a 45 minute treatment with Regeneration Solution.
Lane 8: PCR amplification of eluate DNA after a 60 minute treatment with Regeneration Solution

Excellent Recovery of Plasmid DNA after Purification on Regenerated Columns

Overnight cultures of DH5α cells containing pET22b(+) plasmid were lysed by alkaline lysis and applied to silica miniprep columns that were not regenerated, regenerated only one time, or regenerated 5 times. Plasmid DNA was eluted with 100µL of 10mM Tris-HCl, pH 8.5 and DNA yields were determined by absorbance at 260nm.

Related Products

  • Ready PCR Mix, 2X (N806)
  • Phenol:Chloroform pH 6.7/8.0 (0883)
  • Proteinase K (0706) 
  • RNAse A, Pancreatic (0675)
  • Agarose I (0710)